| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lancefieldella parvula | TGTTTTGGGCAGCGATGC | ACCTCTTCACGCTGCATGT | 59.35 | 59.63 | 225 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Lancefieldella parvula | ACCAAGCGGTGTTGCAAC | ACACTTGTGGCGACCTGT | 59.20 | 59.09 | 204 | 148 |
100.00%
|
100.00%
|
| Primer 3 | Lancefieldella parvula | AAAGCACTCACCCCAGAGC | TGGACCACGTAAGACCACC | 59.93 | 58.95 | 187 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Lancefieldella parvula | ACCAAGCGGTGTTGCAAC | TGAAGCTCACGGAAGGCAG | 59.20 | 60.00 | 246 | 140 |
100.00%
|
100.00%
|
| Primer 5 | Lancefieldella parvula | AAGTGGACTTGGCTCCAGC | TCTTCGAGATCGCGAGCA | 59.93 | 58.50 | 153 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Lancefieldella parvula | TGGCACGTCAAGTTGGCA | CGTATCCCAAGGAAGCGCA | 60.12 | 60.15 | 255 | 138 |
100.00%
|
50.00%
|
| Primer 7 | Lancefieldella parvula | TCACGCAGAAACGGTGCA | TGCGCTCGATAACAGGCA | 60.20 | 59.42 | 182 | 138 |
100.00%
|
50.00%
|
| Primer 8 | Lancefieldella parvula | TCACGCAGAAACGGTGCA | ATGCGCTCGATAACAGGCA | 60.20 | 59.86 | 183 | 138 |
100.00%
|
50.00%
|
| Primer 9 | Lancefieldella parvula | TCACGCAGAAACGGTGCA | AGGCTCAACTTCAACGGCA | 60.20 | 59.85 | 238 | 138 |
100.00%
|
50.00%
|
| Primer 10 | Lancefieldella parvula | ACCAAGCGGTGTTGCAAC | GTCGCTTTCCCTCAGCCAT | 59.20 | 60.08 | 188 | 138 |
100.00%
|
100.00%
|